Bovine Viral Diarrhea Virus Antigen Test

The BVDV Ag Test is a lateral flow immunochromatographic assay for the qualitative detection of bovine viral diarrhea virus antigen (BVDV Ag) in bovine ear-notch (ear-tissue biopsy) specimen.

Specification

Intended Use:detection of bovine viral diarrhea virus antigen

Principle: lateral flow immunochromatographic assay.

Specimen: bovine ear-notch (ear-tissue biopsy)

Materials provided:

10 test cards

10 disposable droppers
10 vials of assay buffer
1 package insert

Test Procedure

1. Collect cattle's ear-notch (ear-tissue biopsy) specimen from the ear and ear notch must measure at least 1cm in diameter.
-Short Term: Ear notches can be stored dry at 2–8°C. If refrigerated storage is not available, ear notches can be stored dry at 18–30°C for 3 days.
-Long Term: If prolonged storage is necessary, the ear notch can be stored frozen at or below -20°C.

2. Allow all materials, including specimen and test device, recover to 15-25°C before running the assay.

3. Take out the test device from the foil pouch and place it horizontally.

4. Add the specimen into the assay buffer tube and soak the ear notch in the tube at 18-30°C for 15minutes (range: 15minutes -3 days).

5. Place 4 drops into the sample well "S" of the test device.

6. Interpret the result in 5-10 minutes. Result after 15 minutes is considered as invalid.

Interpretation of Results

Positive (+): The presence of both "C" line and zone "T" line, no matter T line is clear or vague.

Negative (-): Only clear C line appears. No T line.

Invalid: No colored line appears in C zone. No matter if T line appears.

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