Equine Rotavirus Antigen Test

The Equine Rotavirus Ag Test is a lateral flow immunochromatographic assay designed for detection of equine rotavirus antigen (ROTA Ag) in equine feces. It offers two options for result reading: visual judgment or semi-quantitative analysis with the FIC-Q100N Dry Immunoassay Analyzer (required only for Method 2).

 

 

Test Procedure

 

Test procedures of visual interpretation

1. Remove the test card from the foil pouch and place it flat on a work surface.
2. Transfer 4 drops of the treated sample from the assay buffer tube into the sample well.
3. Start the timer. Wait for 5 to 10 minutes to read the result.

 

Interpretation of visual interpretation results

-Positive (+): Both the Control (C) line and Test (T) line appear as magenta-colored lines in the testing window. Any faintly visible T line -should be considered positive when the C line is also present.
-Negative(-): The C line is visible, but the T line is not visible.
-Invalid: No line appears in the C zone.

 

Test procedures of instrumental interpretation (Standard Test)

1. Turn on the FIC-Q100N Dry Immunoassay Analyzer.
2. Use the analyzer's scanner to scan the QR code on the inner label of the box or the label of bag to import the calibration curve into the analyzer.
3. Remove the test card from the foil pouch.
4. Position the front of the card facing up, ensuring that the sample well is oriented towards the outside of the analyzer's test card slot.
5. Insert the card into the test card slot of the analyzer.
6. Transfer 4 drops of the treated sample from the assay buffer tube into the sample well of the test card.
7. Push the test card to the farthest end of the slot.
8. Tap "Standard Test" on the screen to start the testing. Please refer to the analyzer's operation manual for detailed instructions.

 

Test procedures of instrumental interpretation (Quick Test)

1. Turn on the FIC-Q100N Dry Immunoassay Analyzer .
2. Use the analyzer's scanner to scan the QR code on the inner label of the box or the label of bag to import the calibration curve into the analyzer.
3. Remove the test card from the foil pouch.
4. Transfer 4 drops (using a disposable dropper) of the treated sample into the sample well.
5. Wait for 5-10 minutes, then push the test card to the farthest end of the test card slot.
6. Tap "Quick Test" on the screen to start the testing. Please refer to the analyzer's operation manual for detailed instructions.

 

 

 

Order Information

Product No.	Product Name	Storage Temperature	Package Unit
VD574Q	Equine Rotavirus Antigen Test	2-30°C	5 Tests/Box