Leptospira Spp. Antibody Test

Used for:Leptospira spp. Antibody Test
Model No:VD602Q
Abbr.:LEPTO Ab
Assay Time:5-10 minutes
Specimen:serum, plasma or whole blood

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VD602Q LEPTO Ab PI 7010035801

Description

The Leptospira spp. Ab Test is a lateral flow immunochromatographic assay designed for detection of Leptospira spp. antibodies (LEPTO Ab) in equine serum, plasma, or whole blood. It offers two options for result reading: visual judgment or semi-quantitative analysis with the FIC-Q100N Dry Immunoassay Analyzer (required only for Method 2).

Specification

Intended Use: detection of Leptospira spp. antibodies

Principle: lateral flow immunochromatographic assay.

Specimen: equine serum, plasma or whole blood

Materials provided:

5 LEPTO Ab test cards
5 Vials of assay buffer
5 Disposable droppers
5 Capillary droppers
1 Instruction for use

Test Procedure

Test procedures of visual interpretation

1. Remove the test card from the foil pouch and place it flat on a work surface.
2. Transfer 1 drop (using a capillary dropper) of the treated sample into the sample well.
3. Transfer 3 drops (using a disposable dropper) of the assay buffer into the sample well.
4. Start the timer. Wait for 5 to 10 minutes to read the result.

Interpretation of visual interpretation results

-Positive (+): Both the Control (C) line and Test (T) line appear as magenta-colored lines in the testing window. Any faintly visible T line -should be considered positive when the C line is also present.
-Negative(-): The C line is visible, but the T line is not visible.
-Invalid: No line appears in the C zone.

Test procedures of instrumental interpretation (Standard Test)

Leptospira spp. Antibody Test

1. Turn on the FIC-Q100N Dry Immunoassay Analyzer.
2. Use the analyzer's scanner to scan the QR code on the inner label of the box or the label of bag to import the calibration curve into the analyzer.
3. Remove the test card from the foil pouch.
4. Position the front of the card facing up, ensuring that the sample well is oriented towards the outside of the analyzer's test card slot.
5. Insert the card into the test card slot of the analyzer.
6. Use a pipette with a range of 2-20 μL to place 10 μL of the prepared sample into the sample well. Then, use a pipette with a range of 10-100 μL to place 90 μL of the assay buffer into the sample well.
7. Push the test card to the farthest end of the test card slot.
8. Tap "Standard Test" on the screen to start the testing. Please refer to the analyzer's operation manual for detailed instructions.

Test procedures of instrumental interpretation (Quick Test)

Leptospira spp. Antibody Test

1. Turn on the FIC-Q100N Dry Immunoassay Analyzer .
2. Use the analyzer's scanner to scan the QR code on the inner label of the box or the label of bag to import the calibration curve into the analyzer.
3. Remove the test card from the foil pouch.
4. Transfer 1 drop (using a capillary dropper) of the treated sample into the sample well.
5. Transfer 3 drops (using a disposable dropper) of the assay buffer into the sample well.
6. Wait for 5-10 minutes, then push the test card to the farthest end of the test card slot.
7. Tap "Quick Test" on the screen to start the testing. Please refer to the analyzer's operation manual for detailed instructions.

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