Pay attention to three points to do the experiment easily

Before each test, a detailed test plan should be listed, including test intent, proposed method, varieties and potency of the primary and secondary antibodies used, planned operational procedures and expected test results, etc. The test group number was marked on the glass slides with a glass pen. Positive and negative controls should be matched for each antibody.

1. Purchaser's concentrated antibodies are generally required to be divided into multiple ampoules to prevent repeated freezing and thawing failure. Each ampoule contains 10 to 20rA according to the required amount. Except for keeping one for current use, the rest should be stored in a low temperature refrigerator immediately (below -20℃). Take one when you use it. In order to avoid long-term preservation in the 4℃ refrigerator failure.

2. Sterility and Sterilization Keeping antibodies in use sterile is difficult, but objects in contact with antibodies such as droppers, pipettes, test tubes, ampoules, and samplers are sterilized to minimize the chance of contamination. Otherwise, the antibody is easy to contaminate bacteria and fungi, and then the rapid occurrence of turbidity deposition and failure.

3. Antibody dilution is generally diluted according to its operating concentration, such as 1:100, 1:1000, etc., and then use a vortex to mix the liquid. The remaining antibodies can be stored in a bucket ℃ refrigerator. Try to use it up in 1-2 months, and do not put it below 0℃. Otherwise, the antibody will freeze quickly, and repeated freeze-thaw can make the antibody titer drop sharply. As for the dilution of antibodies, in summer when the temperature is very high, add a little preservative such as sodium azide or thiosal, so as to avoid the effect on the section for more than 10 hours and the fungus.